Introduction

Life in a Shared Resource Flow Cytometry Laboratory (SRL) or Core Cytometry Facility is very dynamic and sometimes we are often faced with challenging samples. In a biomedical research setting these could be anything from subcellular organelles, such as, nuclei and mitochondria, debris-ridden tissue preps, non-mammalian organisms including plant cells, plankton, as well as, bacteria and viruses. Each of these samples present unique challenges to a cytometrist. The following resource will provide some approaches and tips to working with these samples in both a biomedical and aquatic cytometry facility.

Aquatic and Environmental Samples

• Phytoplankton Enumeration and Cell Sorting
• Small Particle Detection
  • Bacterial Enumeration and Cell sorting
  • Viral Enumeration and Cell Sorting

Biomedical Samples

• Subcellular organelles
  • Nuclei
  • Mitochondrion